Composite

Part:BBa_I716016:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-07-24)


(yfbE-rbs-RFP) jp paper style


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 876
    Illegal AgeI site found at 988
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

make (yfbE-rbs-ATG) + (RFP_no_ATG) from pcr prod

PCR product (RFP_no_ATG), digested BglII/XhoI
Digest I716013 (yfbE-rbs-ATF) BamHI/XhoI (2387+9), L
Ligate, get I716016 (yfbE-rbs-RFP_jp)


or for rfp step, (I716015)  2063+693 get small.


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

PCR product RFP_no_ATG + I716013

References